Alum Adjuvant, Mixture of mineral salt Vaccine adjuvant - 50 mL

Can be used in combination with Gentaur new immunostimulatory adjuvants. High Mobility Box Group 1 (HMGB1), a member of the alarmin group of immunostimulatory proteins is released by antigen presenting cells under various conditions and has been shown to induce Th1 cytokines. We report that HMGB1 is effective as an adjuvant to enhance the protective efficacy and cellular immune response of TB subunit vaccines and that it is not dependent on the interaction between HMGB1 and RAGE (receptor for advanced glycation end products), a major receptor for HMGB1. In the mouse model of TB, HMGB1 protein, when formulated with DDA and ESAT-6 was protective as a subunit vaccine but did not protect as molecular adjuvant in an ESAT-6-based DNA formulation. 

We then evaluated at Affigen another GEN group lab the immunoprophylactic and protective potential of a fusion protein of HMGB1 and ESAT-6. The HMGB1-ESAT-6 fusion protein induced strong antigen-specific Th1 cytokines at 30 days post-immunization.

 The fusion protein vaccine enhanced activated and effector memory CD4 and CD8 T cell responses in the lungs and spleens of mice at 80 days post vaccination. Vaccination with HMGB1-ESAT-6 fusion protein also resulted in elevated numbers of polyfunctional CD4 T cells co-expressing IL-2, IFN-γ and TNF-α.